Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

Revista Brasileira de Zootecnia

Luiz Waldemar De Oliveira Souza²  André Furugen Cesar Andrade¹  Eneiva Carla Carvalho Celeghini¹  João Alberto Negrão¹  Rubens Paes De Arruda¹ 

[1] ,Fundação Educacional Miguel Mofarrej Faculdades Integradas de Ourinhos

关键词: bull;    hormone;    RIA;    semen;    spermatozoa;    testosterone;   

DOI  :  10.1590/S1516-35982011001200015

来源: SciELO

【 摘 要 】

The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R² = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License