Polymorphism of alpha s1-casein gene in a dairy goat herd in the southeastern region of Brazil

Revista Brasileira de Zootecnia

Maria Amélia Menck Soares²  Marcelo Teixeira Rodrigues¹  Giuliana Patrícia Mognol¹  Lucinéia De Fátima Chasko Ribeiro¹  José Luis Da Conceição Silva¹  Rose Meire Costa Brancalhão² 

[1] ,Universidade Estadual do Oeste do Paraná Centro de Ciências Biológicas e da Saúde Cascavel Paraná ,Brasil

关键词: Alpine;    Capra hircus;    CSN1S1;    genotyping;    milk proteins;    Saanen;    Alpina;    Capra hircus;    CSN1S1;    genotipagem;    proteínas do leite;    Saanen;   

DOI  :  10.1590/S1516-35982009000600008

来源: SciELO

【 摘 要 】

Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine the frequencies of major alleles for null, low, intermediate and high milk protein expression in a herd of dairy goats raised in the southeastern region of Brazil. Genomic DNA samples were obtained from leukocytes of 145 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in both agarose (O and E allele) and polyacrylamide gels (F allele). For better characterization of the F allele, a PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) study was performed employing the endonuclease XmnI. The allelic frequencies in the herd of 62 Saanen goats studied were: CSN1S1E = 0.35; CSN1S1F = 0.30; CSN1S1O1 = 0.02; CSN1S1A+B+C = 0.30, other alleles = 0.03. In another group of 83 Alpine animals, the frequencies were: CSN1S1E = 0.48; CSN1S1F = 0.28; CSN1S1O1 = 0.01; CSN1S1A+B+C = 0.20, other alleles = 0.03. In the region of exon 9 and intron downstream, where mutations that characterize the F allele occur, it was verified that different intragenic haplotypes may exist, involving the deletion of the 23rd nucleotide in the ninth exon in addition to the insertion of 11bp on intron. These haplotypes may be used to make direct association with other alleles. Although rare, a higher number of combinations were found in this work by evaluating in conjunction the region of the insertion of 3bp in the referred intron, which may allow a higher number of associations. A complete characterization of these combinations will allow elaborating simplified protocols to identify animals concerning the alleles of CSN1S1 gene in goats.

【 授权许可】

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