Analysis of heparanase isoforms and cathepsin B in the plasma of patients with gastrointestinal carcinomas: analytical cross-sectional study

Sao Paulo Medical Journal

Carina Mucciolo Melo¹  Clarice Silvia Taemi Origassa¹  Thérèse Rachell Theodoro¹  Leandro Luongo Matos¹  Thaís Aguilar Miranda¹  Camila Melo Accardo¹  Rodrigo Ippolito Bouças¹  Eloah Rabello Suarez¹  Madalena Maria Nunes Silva Pares¹  Daniel Reis Waisberg¹  Giovanna Canato Toloi¹  Helena Bonciani Nader¹  Jaques Waisberg¹  Maria Aparecida Silva Pinhal¹ 

关键词: Glucuronidase;    Cathepsin B;    Tumor markers;    biological;    Carcinoma;    Plasma;    Glucuronidase;    Catepsina B;    Marcadores biológicos de tumor;    Carcinoma;    Plasma;   

DOI  :  10.1590/1516-3180.2013.7080003

来源: SciELO

【 摘 要 】

CONTEXT AND OBJECTIVE: Heparanase-1 degrades heparan sulfate and has been correlated with tumor progression. Although the isoform heparanase-2 has no catalytic activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase isoform expression and cathepsin B activity in plasma samples from patients with gastrointestinal carcinomas, compared with healthy individuals (control group). DESIGN AND SETTING: This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools. METHODS: Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured. RESULTS: The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma. CONCLUSION: Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors.

【 授权许可】

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