Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil

Sao Paulo Medical Journal

Andréa Carla De Souza Góes¹  Dayse Aparecida Da Silva¹  Cristiane Santana Domingues¹  João Marreiro Sobrinho¹  Elizeu Fagundes De Carvalho¹ 

[1],Universidade Estadual do Rio de Janeiro Instituto de Biologia DNA Diagnostic Laboratory Rio de Janeiro ,Brazil

关键词: Sexual abuse;    Forensic DNA;    STR;    VNTR;    Paternity;    Abuso sexual;    DNA forense;    STR;    VNTR;    Paternidade;   

DOI  :  10.1590/S1516-31802002000300004

来源: SciELO

【 摘 要 】

CONTEXT: Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains. OBJECTIVE: Identification of a criminal in a rape case with 4 suspects using STR and VNTR DNA analysis. TYPE OF STUDY: Forensic DNA analysis. SETTING: DNA Diagnostic Laboratory, Universidade Estadual do Rio de Janeiro, Brazil. PARTICIPANTS: Blood from 4 suspects and the victim, and skin from the fetus. PROCEDURES: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS: Three of the suspects were excluded and one of them was identified as the biological father of the fetus after typing with CTT and FFv Multiplexes. Complementary DNA typing at 3 VNTR loci was also carried out. CONCLUSIONS: After typing four suspects using 6 STR loci, one of them was identified as the biological father of the fetus. In order to significantly enhance the Combined Paternity Index (PI), complementary DNA typing in 3 VNTR loci was carried out. The included suspect was found to be the biological father with a PI of 412,860 (Probability of Paternity: 99.9997%).

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