Screening of supports for immobilization of commercial porcine pancreatic lipase

Materials Research

Robison Scherer²  J. Vladimir Oliveira²  Sibele Pergher¹  Débora De Oliveira² 

[1],Universidade Regional Integrada do Alto Uruguai e das Missões Departamento de Engenharia de Alimentos Erechim RS ,Brazil

关键词: porcine pancreatic lipase;    immobilization;    screening;    supports;   

DOI  :  10.1590/S1516-14392011005000079

来源: SciELO

【 摘 要 】

The aim of this work is to report the performance of different supports for the immobilization of commercial porcine pancreatic lipase. The immobilization tests were carried out in several types of Accurel, activated alumina, kaolin, montmorillonite, ion exchange resins and zeolites. The characterization of the supports showed differences in terms of specific area and morphology. The characteristics of the supports influenced the amount of enzyme adsorbed, yield of immobilization and esterification activity of the resulting immobilized catalyst. The clays KSF and natural and pillared montmorillonites presented potential for use as support for lipase immobilization in terms of yield and esterification activity. Yields of immobilization of 76.32 and 52.01% were achieved for clays KSF and natural montmorillonite, respectively. Esterification activities of 754.03, 595.51, 591.88 and 515.71 U.g-1 were obtained for lipases immobilized in Accurel MP-100, Amberlite XAD-2, mordenite and pillared montmorillonite, respectively.

【 授权许可】

CC BY   
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