Thrombospondin in protein malnutrition induced hypoplasia

Revista de Nutrição

Cidônia De Lourdes Vituri²  Márcio Alvarez-silva¹  Andréa Gonçalves Trentin¹  Vera Lúcia Cardoso Garcia Tramonte¹  Primavera Borelli¹ 

[1] ,Universidade Federal de Santa Catarina Centro de Ciências da Saúde Departamento de Análises ClínicasFlorianópolis SC ,Brasil

关键词: bone marrow;    extracellular matrix;    hypoplasia;    protein malnutrition;    thrombospondin;    medula óssea;    matriz extracelular;    hipoplasia;    desnutrição protéica;    trombospondina;   

DOI  :  10.1590/S1415-52732005000600003

来源: SciELO

【 摘 要 】

OBJECTIVE: The objective of the present study was to measure the concentration of bone marrow extracellular matrix thrombospondin in mice, following hypoplasia induced by protein malnutrition. METHODS: Two-month-old male Swiss mice were submitted to protein malnutrition by way of a low-protein diet containing 4.0% casein until they lost 20.0% of their original body weight, while the control group mice were fed 14.0% casein for 15 days. The bone marrows of the animals were aspirated and transferred to phosphate-buffered saline tubes for extraction. The extracellular matrix protein was analyzed by 7.5% SDS-PAGE and thrombospondin by Enhanced Chemiluminescence Light Western blotting. RESULTS: The amount of thrombospondin was 30% higher in the undernourished samples when compared to the control samples. CONCLUSION: This study suggests that the hypoplasia induced by protein malnutrition probably alters the functioning of the bone marrow microenvironment resulting in a higher thrombospondin concentration.

【 授权许可】

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