Revista de Nutrição | |
Thrombospondin in protein malnutrition induced hypoplasia | |
Cidônia De Lourdes Vituri2  Márcio Alvarez-silva1  Andréa Gonçalves Trentin1  Vera Lúcia Cardoso Garcia Tramonte1  Primavera Borelli1  | |
[1] ,Universidade Federal de Santa Catarina Centro de Ciências da Saúde Departamento de Análises ClínicasFlorianópolis SC ,Brasil | |
关键词: bone marrow; extracellular matrix; hypoplasia; protein malnutrition; thrombospondin; medula óssea; matriz extracelular; hipoplasia; desnutrição protéica; trombospondina; | |
DOI : 10.1590/S1415-52732005000600003 | |
来源: SciELO | |
【 摘 要 】
OBJECTIVE: The objective of the present study was to measure the concentration of bone marrow extracellular matrix thrombospondin in mice, following hypoplasia induced by protein malnutrition. METHODS: Two-month-old male Swiss mice were submitted to protein malnutrition by way of a low-protein diet containing 4.0% casein until they lost 20.0% of their original body weight, while the control group mice were fed 14.0% casein for 15 days. The bone marrows of the animals were aspirated and transferred to phosphate-buffered saline tubes for extraction. The extracellular matrix protein was analyzed by 7.5% SDS-PAGE and thrombospondin by Enhanced Chemiluminescence Light Western blotting. RESULTS: The amount of thrombospondin was 30% higher in the undernourished samples when compared to the control samples. CONCLUSION: This study suggests that the hypoplasia induced by protein malnutrition probably alters the functioning of the bone marrow microenvironment resulting in a higher thrombospondin concentration.
【 授权许可】
CC BY
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