DNA barcoding in Atlantic Forest plants: what is the best marker for Sapotaceae species identification?

Genetics and Molecular Biology

Caio Vinicius Vivas²  Ramiris César Souza Moraes²  Anderson Alves-araújo¹  Marccus Alves¹  Eduardo Mariano-neto¹  Cássio Van Den Berg¹  Fernanda Amato Gaiott² 

[1] ,Universidade Estadual de Santa Cruz Departamento de Ciências Biológicas Ilhéus BA ,Brazil

关键词: internal transcribed spacer;    taxonomy;    tree species;    tropical forest;   

DOI  :  10.1590/S1415-47572014005000019

来源: SciELO

【 摘 要 】

The Atlantic Forest is a phytogeographic domain with a high rate of endemism and large species diversity. The Sapotaceae is a botanical family for which species identification in the Atlantic Forest is difficult. An approach that facilitates species identification in the Sapotaceae is urgently needed because this family includes threatened species and valuable timber species. In this context, DNA barcoding could provide an important tool for identifying species in the Atlantic Forest. In this work, we evaluated four plant barcode markers (matK, rbcL, trnH-psbA and the nuclear ribosomal internal transcribed spacer region -ITS) in 80 samples from 26 species of Sapotaceae that occur in the Atlantic Forest. ITS yielded the highest average interspecific distance (0.122), followed by trnH-psbA (0.019), matK (0.008) and rbcL (0.002). For species discrimination, ITS provided the best results, followed by matK, trnH-psbA and rbcL. Furthermore, the combined analysis of two, three or four markers did not result in higher rates of discrimination than obtained with ITS alone. These results indicate that the ITS region is the best option for molecular identification of Sapotaceae species from the Atlantic Forest.

【 授权许可】

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