Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study

Genetics and Molecular Biology

Jaroslav A. Hubacek²  Vera Lanska¹  Vera Adamkova¹ 

[1] ,Institute for Clinical and Experimental Medicine Center for Experimental Medicine Prague,Czech Republic

关键词: cholinergic receptors;    polymorphism;    smoking;   

DOI  :  10.1590/S1415-47572014005000023

来源: SciELO

【 摘 要 】

Smoking has a significant heritable component of approximately 30-60%. Recent genome wide association studies have identified single nucleotide polymorphisms (SNPs) within the nicotinic cholinergic receptor subunits 3 (rs578776), 5 (rs16969968) and β3 (rs6474412), which are associated with nicotine dependence in Western European populations. To analyze the association in a Czech population, we genotyped 1,191 males and 1,368 females (post-MONICA study). The WHO protocol was used to examine smoking status and the number of cigarettes smoked per day. There were 32.1% current and 27.6% past smokers among the males and 22.5% current and 13.8% past smokers among the females. We have not confirmed the original results: the SNPs rs16969968 (p = 0.07), rs578776 (p = 0.16) and rs6474412 (p = 0.76) were not associated with smoking status (never-smokers vs. ever-smokers) in the entire population, if a codominant model of analysis was used. This result was valid for both the male and female subpopulations if analyzed separately and adjusted for age. Finally, in ever-smokers, the number of cigarettes smoked per day was also independent of different genotypes, regardless of which polymorphism (and gender) was analyzed (the lowest p value was 0.49). The association between the cholinergic receptors - nicotinic subunits (-3, -5 and -ß3), and smoking behavior may be population-dependent.

【 授权许可】

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