Genetic variation in cultivated Rheum tanguticum populations

Genetics and Molecular Biology

Yanping Hu²  Xiaolong Xie¹  Li Wang²  Huaigang Zhang²  Jian Yang²  Yi Li² 

[1] ,Chinese Academy of Sciences Northwest Institute of Plateau Biology Xining,China

关键词: cultivated populations;    genetic variation;    ISSR;    Polygonaceae;    Rheum tanguticum Maxim. ex Balf;   

DOI  :  10.1590/S1415-47572014000400010

来源: SciELO

【 摘 要 】

To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (Gst = 0.2742) and Bayesian analysis (θB = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.

【 授权许可】

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