Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

Genetics and Molecular Biology

Camille E Granada²  Marcos Strochein¹  Luciano K Vargas¹  Manuela Bruxel¹  Enilson Luiz Saccol De Sá¹  Luciane M.p Passaglia² 

[1] ,Universidade Federal do Rio Grande do Sul Instituto de Biociências Departamento de GenéticaPorto Alegre RS ,Brazil

关键词: Rhizobia;    nodulation;    nitrogen;    specificity;   

DOI  :  10.1590/S1415-47572014000300012

来源: SciELO

【 摘 要 】

This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.

【 授权许可】

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