Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain

Genetics and Molecular Biology

Jie Gong¹  Yinjie Xie¹  Kun Yu¹  Ya'nan Yang¹  Huiyang Huang¹  Haihui Ye¹ 

[1] ,Xiamen University College of Ocean and Earth Sciences Xiamen Fujian ,China

关键词: chymotrypsin;    Scylla paramamosain;    immune response;    immunocytochemistry;    in situ hybridization;   

DOI  :  10.1590/S1415-47572014005000007

来源: SciELO

【 摘 要 】

Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab's immune response.

【 授权许可】

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