Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Genetics and Molecular Biology

Elizabete Cristina Da Silva²  Concepta Margaret Mcmanus²  Maria Pia Souza Lima Mattos De Paiva Guimarães¹  Aurora M.g. Gouveia¹  Olivardo Facó¹  Daniel M. Pimentel¹  Alexandre Rodrigues Caetano²  Samuel Rezende Paiva² 

[1] ,Universidade de Brasília Ciências Animais Brasília DF ,Brazil

关键词: parentage errors;    animal breeding;    animal genetic resources;    Capra hircus;   

DOI  :  10.1590/S1415-47572014000100010

来源: SciELO

【 摘 要 】

Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.

【 授权许可】

CC BY   
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