Gene expression profile analysis of human intervertebral disc degeneration

Genetics and Molecular Biology

Kai Chen¹  Dajiang Wu¹  Xiaodong Zhu¹  Haijian Ni¹  Xianzhao Wei¹  Ningfang Mao¹  Yang Xie¹  Yunfei Niu¹  Ming Li¹ 

[1] ,Changhai Hospital affiliated to the Second Military Medical University Department of OrthopedicsShanghai ,China

关键词: genes;    intervertebral disc degeneration;    molecular classification;    protein-protein interaction;   

DOI  :  10.1590/S1415-47572013000300021

来源: SciELO

【 摘 要 】

In this study, we used microarray analysis to investigate the biogenesis and progression of intervertebral disc degeneration. The gene expression profiles of 37 disc tissue samples obtained from patients with herniated discs and degenerative disc disease collected by the National Cancer Institute Cooperative Tissue Network were analyzed. Differentially expressed genes between more and less degenerated discs were identified by significant analysis of microarray. A total of 555 genes were significantly overexpressed in more degenerated discs with a false discovery rate of < 3%. Functional annotation showed that these genes were significantly associated with membrane-bound vesicles, calcium ion binding and extracellular matrix. Protein-protein interaction analysis showed that these genes, including previously reported genes such as fibronectin, COL2A1 and f-catenin, may play key roles in disc degeneration. Unsupervised clustering indicated that the widely used morphology-based Thompson grading system was only marginally associated with the molecular classification of intervertebral disc degeneration. These findings indicate that detailed, systematic gene analysis may be a useful way of studying the biology of intervertebral disc degeneration.

【 授权许可】

CC BY   
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