In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses

Genetics and Molecular Biology

Rodrigo Juliano Oliveira²  Maria José Sparça Salles¹  Ariane Fernanda Da Silva¹  Tatiane Yumi Nakamura Kanno¹  Ana Carolina Dos Santos Lourenço¹  Véssia Da Silva Leite¹  Hevenilton José Matiazi¹  João Renato Pesarini²  Lúcia Regina Ribeiro¹  Mário Sérgio Mantovani¹ 

[1] ,Universidade Federal de Mato Grosso do Sul Núcleo de Hospital Universitário Centro de Estudos em Célula Tronco, Terapia Celular e Genética ToxicológicaCampo Grande MS ,Brazil

关键词: β-glucan;    cyclophosphamide;    antimutagenicity;    antigenotoxicity;    mice;   

DOI  :  10.1590/S1415-47572013005000028

来源: SciELO

【 摘 要 】

Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage reduction rate between 100-62.04% and 94.34-59.52% for mutagenic and genotoxic damages, respectively. This activity decreased as the treatment was extended. During the sixth week of treatment antimutagenicity rates were reduced to 59.51-39.83% and antigenotoxicity was not effective. This leads to the conclusion that the efficacy of β-glucan in preventing DNA damage is limited when treatment is extended, and that its use as a chemotherapeutic adjuvant need to be better clarified.

【 授权许可】

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