Differential detection of transposable elements between Saccharum species

Genetics and Molecular Biology

Marislane Carvalho Paz De Souza¹  Jéssica Naiana Silva¹  Cícero Almeida¹ 

[1] ,Universidade Federal de Alagoas Laboratório de Recursos Genéticos Arapiraca AL ,Brazil

关键词: sugarcane;    dot-blot hybridization;    LTR retrotransposons;    repetitive DNA;   

DOI  :  10.1590/S1415-47572013005000030

来源: SciELO

【 摘 要 】

Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analyze the repetition of DNA sequences in S. officinarum and S. spontaneum. For this purpose, genomic DNA from S. officinarum was digested with restriction enzymes and the fragments cloned. Sixty-eight fragments, approximately 500 bp, were cloned, sequenced and had their identity analyzed in NCBI, and in the rice, maize, and sorghum genome databases using BLAST. Twelve clones containing partial transposable elements, one single-copy control, one DNA repetitive clone control and two genome controls were analyzed by DNA hybridization on membrane, using genomic probes from S. officinarum and S. spontaneum. The hybridization experiment revealed that six TEs had a similar repetitive DNA pattern in the genomes of S. officinarum and S. spontaneum, while six TEs were more abundant in the genome of S. officinarum. We concluded that the species S. officinarum and S. spontaneum have differential accumulation LTR retrotransposon families, suggesting distinct insertion or modification patterns.

【 授权许可】

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