Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae)

Genetics and Molecular Biology

Ebenézer C.s. Bernardes²  Ana M. Benko-iseppon¹  Santelmo Vasconcelos¹  Reginaldo Carvalho¹  Ana C. Brasileiro-vidal¹ 

[1],Instituto Federal de Alagoas Departamento de Ciências da Natureza Maceió AL ,Brazil

关键词: compositae;    Cichorieae;    CMA/DAPI;    rDNA sites;    chromosome rearrangements;   

DOI  :  10.1590/S1415-47572013005000025

来源: SciELO

【 摘 要 】

Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding, polymorphisms were detected within chicory accessions regarding the number and the distribution of rDNA sites in relation to the most frequent karyotype (two pairs with 45S and one with 5S rDNA). The karyological markers developed allowed karyotypic differentiation between both species, uncovering peculiarities in the number and position of rDNA sites, which suggest chromosome rearrangements, such as translocations in chicory cultivars. The interspecific and intraspecific polymorphisms observed emphasize the potential of karyomorphological evaluations, helping our understanding of the relationships and evolution of the group.

【 授权许可】

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