Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers

Genetics and Molecular Biology

Tatiana Aparecida Voltolin²  Manolo Penitente²  Bruna Bueno Mendonça²  José Augusto Senhorini¹  Fausto Foresti¹  Fábio Porto-foresti² 

[1] ,Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Ciências Departamento Ciências BiológicasBauru SP ,Brazil

关键词: AgNOR;    C-banding;    hydrographic basins;    conserved karyotype;    FISH;   

DOI  :  10.1590/S1415-47572013000300008

来源: SciELO

【 摘 要 】

The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosomal DNA probes in five species of the Prochilodus genus (Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus and Prochilodus nigricans) collected from different Brazilian hydrographic basins. The results revealed conservatism in chromosome number, morphology, AgNORs 18S and 5S rDNAs location and constitutive heterochromatin distribution patterns. The minor differences observed in this work, such as an Ag-NOR on a P. argenteus chromosome and a distinct C-banding pattern in P. lineatus, are not sufficient to question the conservatism described for this group. Future work using repetitive DNA sequences as probes for FISH will be interesting to further test the cytogenetic conservatism in Prochilodus.

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