Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots

Genetics and Molecular Biology

Suzane A. Souza²  Adelica A. Xavier¹  Márcia R. Costa¹  Acleide M.s. Cardoso¹  Marlon C.t. Pereira¹  Silvia Nietsche¹ 

[1] ,Universidade Estadual do Norte Fluminense Darcy Ribeiro Centro de Biociências e Biotecnologia Laboratório de BiotecnologiaCampo dos Goytacazes RJ ,Brazil

关键词: ARDRA;    Bacillus sp.;    nifH gene;    rep-PCR;    16S rDNA;   

DOI  :  10.1590/S1415-47572013000200016

来源: SciELO

【 摘 要 】

The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences. Sequence analyses revealed that the isolates were distributed among ten bacterial genera (Agrobacterium, Aneurinibacillus, Bacillus, Enterobacter, Klebsiella, Lysinibacillus, Micrococcus, Paenibacillus, Rhizobium and Sporolactobacillus) and included 15 species. The greatest number of isolates belonged to the genus Bacillus. The bacteria identified in this study may be involved in promoting growth, phosphate solubilization, biological control and nitrogen fixation in bananas.

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