Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease

Genetics and Molecular Biology

Lauro Bücker Neto²  Rafael Rodrigues De Oliveira²  Beatriz Wiebke-strohm²  Marta Bencke²  Ricardo Luís Mayer Weber²  Caroline Cabreira²  Ricardo Vilela Abdelnoor¹  Francismar Correa Marcelino¹  Maria Helena Bodanese Zanettini²  Luciane Maria Pereira Passaglia² 

[1] ,Universidade Federal do Rio Grande do Sul Instituto de Biociências Departamento de GenéticaPorto Alegre RS ,Brazil

关键词: fungal disease;    HyPRP genes;    Glycine max;    real time RT-qPCR;   

DOI  :  10.1590/S1415-47572013005000017

来源: SciELO

【 摘 要 】

Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.

【 授权许可】

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