Identification of RAPD and SCAR markers associated with yield traits in the Indian tropical tasar silkworm Antheraea mylitta drury

Genetics and Molecular Biology

Suhrid R. Dutta²  Prasanta K. Kar¹  Ashok K. Srivastava¹  Manoj K. Sinha¹  Jai Shankar²  Ananta K. Ghosh² 

[1],Indian Institute of Technology Kharagpur Department of Biotechnology Kharagpur,India

关键词: Antheraea mylitta;    quantitative trait;    RAPD;    SCAR marker;    tasar silkworm;   

DOI  :  10.1590/S1415-47572012005000059

来源: SciELO

【 摘 要 】

The tropical tasar silkworm, Antheraea mylitta, is a semi-domesticated vanya silk-producing insect of high economic importance. To date, no molecular marker associated with cocoon and shell weights has been identified in this species. In this report, we identified a randomly amplified polymorphic DNA (RAPD) marker and examined its inheritance, and also developed a stable diagnostic sequence-characterized amplified region (SCAR) marker. Silkworms were divided into groups with high (HCSW) and low (LCSW) cocoon and shell weights, and the F2 progeny of a cross between these two groups were obtained. DNA from these silkworms was screened by PCR using 34 random primers and the resulting RAPD fragments were used for cluster analysis and discriminant function analysis (DFA). The clustering pattern in a UPGMA-based dendogram and DFA clearly distinguished the HCSW and LCSW groups. Multiple regression analysis identified five markers associated with cocoon and shell weights. The marker OPW16(905 bp) showed the most significant association with cocoon and shell weights, and its inheritance was confirmed in F2 progeny. Cloning and sequencing of this 905 bp fragment showed 88% identity between its 134 nucleotides and the Bmc-1/Yamato-like retroposon of A. mylitta. This marker was further converted into a diagnostic SCAR marker (SCOPW 16(826 bp)). The SCAR marker developed here may be useful in identifying the right parental stock of tasar silkworms for high cocoon and shell weights in breeding programs designed to enhance the productivity of tasar silk.

【 授权许可】

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