Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts

Genetics and Molecular Biology

Shumin Zhang¹  Kai Xiong¹  Zhourui Xie¹  Wenting Nan¹  Honglin Liu¹  Jie Chen¹ 

[1] ,Nanjing Agricultural University College of Animal Science and Technology Nanjing,China

关键词: RNAi;    shRNA;    lentivirus;    BLG;    goat;   

DOI  :  10.1590/S1415-47572012005000042

来源: SciELO

【 摘 要 】

β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.

【 授权许可】

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