Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Genetics and Molecular Biology

Ashwin A. Raut²  Anil Kumar¹  Sheo N. Kala¹  Vinod Chhokar¹  Neeraj Rana¹  Vikas Beniwal¹  Sundeep Jaglan¹  Sachin K. Samuchiwal¹  Jitender K. Singh¹  Anamika Mishra² 

[1] ,High Security Animal Disease LaboratoryMadhya Pradesh,India

关键词: buffalo;    DGAT1;    PCR-SSCP;    polymorphism;    SNP;   

DOI  :  10.1590/S1415-47572012005000043

来源: SciELO

【 摘 要 】

Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo.

【 授权许可】

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