Silencing of the integrin-linked kinase gene suppresses the proliferation, migration and invasion of pancreatic cancer cells (Panc-1)

Genetics and Molecular Biology

Xiang-yu Zhu¹  Ning Liu¹  Wei Liu¹  Shao-wei Song¹  Ke-jian Guo¹ 

[1] ,The First Affiliated Hospital of China Medical University Department of Pancreatic and Gastrointestinal Surgery Shenyang Liaoning Province ,China

关键词: E-cadherin;    epithelial-mesenchymal transition (EMT);    integrin-linked kinase (ILK);    Panc-1 cell line;    RNA interference (RNAi);   

DOI  :  10.1590/S1415-47572012005000028

来源: SciELO

【 摘 要 】

Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine-threonine protein kinase that is involved in the regulation of integrin-mediated processes such as cancer cell proliferation, migration and invasion. In this study, we examined the effect of a lentivirus-mediated knockdown of ILK on the proliferation, migration and invasion of pancreatic cancer (Panc-1) cells. Immunohistochemical staining showed that ILK expression was enhanced in pancreatic cancer tissue. The silencing of ILK in human Panc-1 cells led to cell cycle arrest in the G0/G1 phase and delayed cell proliferation, in addition to down-regulating cell migration and invasion. The latter effects were mediated by up-regulating the expression of E-cadherin, a key protein in cell adhesion. These findings indicate that ILK may be a new diagnostic marker for pancreatic cancer and that silencing ILK could be a potentially useful therapeutic approach for treating pancreatic cancer.

【 授权许可】

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