Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries

Genetics and Molecular Biology

Lorrayne Gomes Molina¹  Guilherme Cordenonsi Da Fonseca¹  Guilherme Loss De Morais¹  Luiz Felipe Valter De Oliveira¹  Joseane Biso De Carvalho¹  Franceli Rodrigues Kulcheski¹  Rogerio Margis¹ 

[1] ,Universidade Federal do Rio Grande do Sul Laboratório de Genomas e Populações de Plantas Centro de BiotecnologiaPorto Alegre RS ,Brazil

关键词: next generation sequencing;    small RNA;    siRNA;    molecular markers;   

DOI  :  10.1590/S1415-47572012000200010

来源: SciELO

【 摘 要 】

A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that soybean plants establish with microorganisms that may affect, directly or indirectly, plant health and crop production.

【 授权许可】

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