Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine)

Genetics and Molecular Biology

Paulo Sérgio Schlögl²  André Luis Wendt Dos Santos¹  Leila Do Nascimento Vieira²  Eny Iochevet Segal Floh¹  Miguel Pedro Guerra² 

[1] ,Universidade Federal de Santa Catarina Departamento de Fitotecnia Laboratório de Fisiologia do Desenvolvimento e Genética VegetalFlorianópolis SC ,Brazil

关键词: seed development;    Brazilian Pine;    embryogenesis-regulating genes;    zygotic embryogenesis;   

DOI  :  10.1590/S1415-47572012005000005

来源: SciELO

【 摘 要 】

Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development and germination. All the eight were found to be differentially expressed in the various developmental stages of zygotic embryos, seeds and seedling tissues. To our knowledge, this is the first report on embryogenesis-regulating gene expression in members of the Araucariaceae family, as well as in plants with recalcitrant seeds.

【 授权许可】

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