Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1

Genetics and Molecular Biology

Yue-feng Du²  Liang Liang²  Ying Shi¹  Qing-zhi Long²  Jin Zeng²  Xin-yang Wang²  Da-lin He² 

[1],Xi'an Jiaotong University First Affiliated Hospital of Medical School Department of Urology,China

关键词: prostate cancer;    DNA methylation;    DNMT3;    RNA interference;   

DOI  :  10.1590/S1415-47572012005000021

来源: SciELO

【 摘 要 】

Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

【 授权许可】

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