Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata

Genetics and Molecular Biology

Amirul Firdaus Jamaluddin Jamsari¹  Tan Min-pau¹  Mohd Nor Siti-azizah¹ 

[1] ,Universiti Sains Malaysia School of Biological Sciences Malaysia

关键词: Channa striata;    population genetics;    microsatellite;    multiplex PCR;   

DOI  :  10.1590/S1415-47572011005000007

来源: SciELO

【 摘 要 】

Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.

【 授权许可】

CC BY   
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