Microsatellite characterization of Cimarron Uruguayo dogs

Genetics and Molecular Biology

Rosa Gagliardi²  Llambí Silvia²  Cristina García¹  María Victoria Arruga¹ 

[1] ,Universidad de la República Facultad de Veterinaria Área GenéticaMontevideo,Uruguay

关键词: Cimarron Uruguayo dog;    genetic characterization;    microsatellites;   

DOI  :  10.1590/S1415-47572010005000101

来源: SciELO

【 摘 要 】

Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values > 0.7, in agreement with results for other canine breeds. The PE values for the markers were within the ranges previously described and were generally greater for microsatellites with higher PIC values. The heterozygosity value (0.649) was considered high since only nine microsatellites were analyzed. Compared with data for other breeds, the results obtained here indicate that Cimarron Uruguayo dogs have high genetic diversity.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License