Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)

Genetics and Molecular Biology

Mara Garcia Tavares¹  Carlos Roberto Carvalho¹  Fernanda Aparecida Ferrari Soares¹  Anderson Fernandes¹ 

[1] ,Universidade Federal de Viçosa Departamento de Biologia Geral Viçosa MG ,Brazil

关键词: cytogenetic;    flow cytometry;    genome size;    karyotype;    stingless bee;   

DOI  :  10.1590/S1415-47572010000300019

来源: SciELO

【 摘 要 】

When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.

【 授权许可】

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