High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae)

Genetics and Molecular Biology

Roberto Tarazi²  Maria Andréia Moreno²  Flávio Bertin Gandara²  Elza Martins Ferraz²  Mário Luiz Teixeira Moraes¹  Christina Cleo Vinson¹  Ana Yamaguishi Ciampi¹  Roland Vencovsky¹  Paulo Yoshio Kageyama² 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Ciências FlorestaisPiracicaba SP ,Brazil

关键词: Dipteryx alata;    genetic structure;    mating system;    SSR;   

DOI  :  10.1590/S1415-47572010005000007

来源: SciELO

【 摘 要 】

Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds in conservation and breeding programs. Our data indicate that the populations studied had a high degree of genetic diversity and population structure, as suggested by the high level of divergence among populations . The estimated outcrossing rate suggested a mixed mating system, and the intrapopulation fixation index was influenced by SGS. We conclude that seed harvesting for genetic conservation and breeding programs requires a minimum distance between trees of 196 m to avoid collecting seeds from related seed-trees.

【 授权许可】

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