Parentage test in broad-snouted caimans (Caiman latirostris, Crocodylidae) using microsatellite DNA

Genetics and Molecular Biology

Rodrigo B. Zucoloto²  Luciano M. Verdade¹  Priscilla M.s. Villela¹  Luciana C.a. Regitano¹  Luiz L. Coutinho¹ 

[1] ,Universidade Federal da BahiaSalvador BA ,Brazil

关键词: crocodilians;    caiman;    parentage;    microsatellite DNA;   

DOI  :  10.1590/S1415-47572009005000077

来源: SciELO

【 摘 要 】

In this study, microsatellite markers, developed for Alligator mississipiensis and Caiman latirostris, were used to assess parentage among individuals from the captive colony of Caiman latirostris at the University of São Paulo, in Piracicaba, São Paulo, Brazil. Many of the females in the colony were full siblings, which made maternal identification difficult due to genotypic similarity. Even so, the most likely mother could be identified unambiguously among offspring in most of the clutches studied. Two non-parental females displayed maternal behavior which would have misled managers in assigning maternity based on behavior alone. This set of variable loci demonstrates the utility of parentage testing in captive propagation programs.

【 授权许可】

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