Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP

Genetics and Molecular Biology

Miroslav Baránek¹  Jana Raddová¹  Bretislav Krizan¹  Miroslav Pidra¹ 

[1] ,Horticulture  Faculty of MUAF Mendeleum Institute Lednice,Czech Republic

关键词: grapevine;    stress;    somaclonal variation;    virus infection;    AFLP;   

DOI  :  10.1590/S1415-47572009005000079

来源: SciELO

【 摘 要 】

The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes, those subjected to in vitro thermotherapy and virus-infected plants.

【 授权许可】

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