New occurrence of B chromosomes in Partamona helleri (Friese, 1900) (Hymenoptera, Meliponini)

Genetics and Molecular Biology

Cinthia Caroline Cardoso Martins²  Olivia Maria Pereira Duarte²  Ana Maria Waldschmidt¹  Rogério Marco De Oliveira Alves¹  Marco Antônio Costa² 

[1] ,Universidade Estadual de Santa Cruz Departamento de Ciências Biológicas Ilhéus BA ,Brazil

关键词: stingless Bee;    Partamona helleri;    geographic variation;    supernumerary chromosomes;   

DOI  :  10.1590/S1415-47572009005000065

来源: SciELO

【 摘 要 】

Cytogenetic analyses of the stingless bee Partamona helleri collected in the state of Bahia, Northeast Brazil revealed the chromosome numbers n = 18 in the haploid males and 2n = 35 in the diploid females. All karyotypes displayed one large acrocentric B chromosome, which differs from the minute B chromosomes previously described in the populations from southeastern Brazil. Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining also revealed a remarkable interpopulational divergence regarding both the regular karyotype and the B chromosomes. The B chromosomes found in the samples from Jequié, Bahia, were entirely heterochromatic, while those found in Cravolândia, Bahia, displayed a euchromatic portion at the telomeric end of the long arm. CMA3 labeling sites varied from seven to eight between the two localities in Bahia, due to the presence of an extra GC-rich block in the karyotype of the samples from Jequié. This is the first report of a large B chromosome in P. helleri and reveals the occurrence of a geographic differentiation within this species.

【 授权许可】

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