Transferability and characterization of microsatellite markers in two Neotropical Ficus species

Genetics and Molecular Biology

Alison Gonçalves Nazareno²  Rodrigo Augusto Santinelo Pereira¹  Juliana Massimino Feres¹  Moacyr Antônio Mestriner¹  Ana Lilia Alzate-marin¹ 

[1] ,Universidade de São Paulo Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Programa de Pós-graduação em Biologia ComparadaRibeirão Preto SP ,Brazil

关键词: ecological genetics;    Ficus citrifolia;    Ficus eximia;    Moraceae;    SSRs;   

DOI  :  10.1590/S1415-47572009005000056

来源: SciELO

【 摘 要 】

Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.

【 授权许可】

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