Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers

Genetics and Molecular Biology

Ivan Schuster²  Elisa Serra Negra Vieira²  Glacy Jaqueline Da Silva¹  Francisco De Assis Franco²  Volmir Sérgio Marchioro² 

[1] ,Cooperativa Central de Pesquisa AgrícolaCascavel PR ,Brazil

关键词: Triticum aestivum;    germplasm;    cultivar characterization;    cluster analysis;    molecular markers;   

DOI  :  10.1590/S1415-47572009005000045

来源: SciELO

【 摘 要 】

Wheat (Triticum aestivum) is one of the most important food staples in the south of Brazil. Understanding genetic variability among the assortment of Brazilian wheat is important for breeding. The aim of this work was to molecularly characterize the thirty-six wheat cultivars recommended for various regions of Brazil, and to assess mutual genetic distances, through the use of microsatellite markers. Twenty three polymorphic microsatellite markers (PMM) delineated all 36 of the samples, revealing a total of 74 simple sequence repeat (SSR) alleles, i.e. an average of 3.2 alleles per locus. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.20 to 0.79, with a mean of 0.49. Genetic distances among the 36 cultivars ranged from 0.10 (between cultivars Ocepar 18 and BRS 207) to 0.88 (between cultivars CD 101 and Fudancep 46), the mean distance being 0.48. Twelve groups were obtained by using the unweighted pair-group method with arithmetic means analysis (UPGMA), and thirteen through the Tocher method. Both methods produced similar clusters, with one to thirteen cultivars per group. The results indicate that these tools may be used to protect intellectual property and for breeding and selection programs.

【 授权许可】

CC BY   
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