Expression of Msx1 and Dlx1 during Dumbo rat head development: correlation with morphological features

Genetics and Molecular Biology

Suhair Katerji²  Nathalie Vanmuylder²  Michal Svoboda¹  Marcel Rooze²  Stéphane Louryan² 

[1] ,Université Libre de Bruxelles Faculté de Médecine Laboratory of Anatomy and EmbryologyBrussels Belgium

关键词: Dumbo rat;    Msx1;    Dlx1;    face;    embryo;    development;   

DOI  :  10.1590/S1415-47572009005000041

来源: SciELO

【 摘 要 】

The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with the aim of understanding their possible role in abnormal craniofacial morphogenesis and examining the possibility of using Dumbo rat as an experimental model for understanding abnormal craniofacial development. We studied the expression of these genes during craniofacial morphogenesis by RT-PCR method. We used Dumbo embryos at E12 and E14 and included the Wistar strain as a control. Semi-quantitative PCR analysis demonstrated that Msx1 and Dlx1 are expressed differently between Dumbo and Wistar rats, indicating that their low expression may underly the Dumbo phenotype.

【 授权许可】

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