Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

Genetics and Molecular Biology

Fábio Pinheiro²  Clarisse Palma-silva¹  Fábio De Barros¹  Salvatore Cozzolino¹ 

[1] ,Universidade de São Paulo Instituto de Biociências Departamento de BotânicaSão Paulo SP ,Brazil

关键词: Epidendrum;    Orchidaceae;    short tandem repeat;    cross-amplification;   

DOI  :  10.1590/S1415-47572009005000037

来源: SciELO

【 摘 要 】

In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.

【 授权许可】

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