Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)

Genetics and Molecular Biology

Guilherme Messias Da Silva²  Edgar Guimarães Bione¹  Diogo Cavalcanti Cabral-de-mello²  Rita De Cássia De Moura¹  Zilá Luz Paulino Simões¹  Maria José De Souza² 

[1] ,Universidade Federal de Pernambuco Departamento de Genética Centro de Ciências BiológicasRecife PE ,Brazil

关键词: chromosome rearrangements;    heterochromatin;    karyotype;    NORs;    FISH;   

DOI  :  10.1590/S1415-47572009005000040

来源: SciELO

【 摘 要 】

Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.

【 授权许可】

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