Estimating genomic instability mediated by Alu retroelements in breast cancer

Genetics and Molecular Biology

Ana Cristina Fazza²  Flavia Cal Sabino²  Nathalia De Setta²  Newton Antonio Bordin Jr.¹  Eloiza Helena Tajara Da Silva¹  Claudia Marcia Aparecida Carareto² 

[1] ,Universidade Estadual Paulista Júlio De Mesquita Filho Instituto de Biociências, Letras e Ciências Exatas Departamento de BiologiaSão José Rio Preto SP ,Brazil

关键词: Alu-PCR;    breast cancer;    gene insertions;    gene deletions;    invasive ductal carcinoma;    recombination;   

DOI  :  10.1590/S1415-47572009005000018

来源: SciELO

【 摘 要 】

Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification.

【 授权许可】

CC BY   
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