Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene

Genetics and Molecular Biology

Jomuna V. Choudhuri¹  Monica B. Mathor¹  Flávia H. Silva¹  Sang W. Han¹ 

关键词: BALB/MK keratinocytes;    epidermal growth factor;    retroviral vector;   

DOI  :  10.1590/S1415-47572008005000009

来源: SciELO

【 摘 要 】

Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time- and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.

【 授权许可】

CC BY   
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