HOXgene analysis in the osteogenic differentiation of human mesenchymal stem cells

Genetics and Molecular Biology

Song Wha Chae¹  Bo Keun Jee¹  Joo Yong Lee¹  Chang Whan Han¹  Yang-whan Jeon¹  Young Lim¹  Kweon-haeng Lee¹  Hyoung Kyun Rha¹  Gue-tae Chae¹ 

关键词: human mesenchymal stem cells;    HOXgenes;    osteogenic differentiation;    stem cell differentiation;    gene profiling;   

DOI  :  10.1590/S1415-47572008005000019

来源: SciELO

【 摘 要 】

Human bone marrow-derived mesenchymal stem cells (hMSCs) have the capacity to differentiate into osteoblasts during osteogenesis. Several studies attempted to identify osteogenesis-related genes in hMSCs. AlthoughHOXgenes are known to play a pivotal role in skeletogenesis, their function in the osteogenesis of hMSCs has not yet been investigated in detail. Our aim was to characterize the expression of 37HOXgenes by multiplex RT-PCR to identify the ones most probably involved in osteogenic differentiation. The results showed that the expression patterns of fourHOXgenes were altered during this process. In particular, the expression levels ofHOXC13andHOXD13were dramatically changed. Real-time PCR and Western blot analysis were performed in order to further analyze the expression ofHOXC13andHOXD13 . The qRT-PCR results showed that transcription ofHOXC13was up-regulated by up to forty times, whereas that ofHOXD13was down-regulated by approximately five times after osteogenic differentiation. The Western blot results for the HOXC13 and HOXD13 proteins also corresponded well with the real-time PCR result. These findings suggest that HOXC13 and HOXD13 might be involved in the osteogenic differentiation of hMSCs.

【 授权许可】

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