Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region

Genetics and Molecular Biology

Aline R. Rabello²  Paulo R. Queiroz²  Kenya C.c. Simões²  Cássia O. Hiragi²  Luzia H.c. Lima¹  Maria Regina V. Oliveira¹  Angela Mehta¹ 

[1] ,Universidade de BrasíliaBrasília DF ,Brazil

关键词: whitefly;    ITS rDNA;    genetic diversity;    phylogenetic analysis;   

DOI  :  10.1590/S1415-47572008000300029

来源: SciELO

【 摘 要 】

The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.

【 授权许可】

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