Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin

Genetics and Molecular Biology

Milena Schenkel Homrich²  Luciane Maria Pereira Passaglia²  Jorge Fernando Pereira¹  Paulo Fernando Bertagnolli¹  Giancarlo Pasquali¹  Mohsin Abbas Zaidi¹  Illimar Altosaar¹  Maria Helena Bodanese-zanettini² 

[1] ,Universidade Federal do Rio Grande do Sul Instituto de Biociências Departamento de GenéticaPorto Alegre RS ,Brazil

关键词: Anticarsia gemmatalis;    cry1Ac gene;    IAS5 cultivar;    insect resistance;    transgenic soybean;   

DOI  :  10.1590/S1415-47572008000300020

来源: SciELO

【 摘 要 】

Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed embryos and all T0, T1, T2 and T3 plants were confirmed by Gus activity, PCR, Southern and western blot, and ELISA techniques. Two T0 plants out of the seven co-transformed plants produced seeds and were analyzed for patterns of integration and inheritance until the T3 generation. Bioassays indicated that the transgenic plants were highly toxic to the velvetbean caterpillar Anticarsia gemmatalis, thus offering a potential for effective insect resistance in soybean.

【 授权许可】

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