DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse

Genetics and Molecular Biology

Fabiana Tavares Pires De Souza Sereno²  José Robson Bezerra Sereno¹  Jose Luis Vega-pla¹  Juan Vicente Delgado¹ 

[1] ,Universidade de Brasília Faculdade de Agronomia e Medicina Veterinária Brasília DF ,Brazil

关键词: Pantaneiro horse;    animal conservation;    microsatellites;    markers;    parentage verification;   

DOI  :  10.1590/S1415-47572008000100013

来源: SciELO

【 摘 要 】

We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (> 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of the Pantaneiro horse using genetic markers was efficient in detecting mistakes during paternity and maternity designation and is an important tool which can be used together with traditional systems of animal identification. The use of genetic markers is recommended in the systematic control of the genealogical registrations and conservation plans to improve genetic aspects of the Pantaneiro horse.

【 授权许可】

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