Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining

Genetics and Molecular Biology

Denilce Meneses Lopes¹  Sílvia Das Graças Pompolo¹  Lúcio Antônio De Oliveira Campos¹  Mara Garcia Tavares¹ 

[1] ,Universidade Federal de Viçosa Departamento de Biologia Geral Viçosa MG ,Brazil

关键词: Melipona;    stingless bees;    B chromosomes;    fluorochromes;    C-banding;   

DOI  :  10.1590/S1415-47572008000100010

来源: SciELO

【 摘 要 】

The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The euchromatin was restricted to the chromosome extremities and was GC-rich, as evidenced by CMA3 staining. The B chromosome was CMA3-negative and DAPI-positive, a heterochromatic constitution similar to that of the A genome chromosomes.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License