Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species

Genetics and Molecular Biology

Adriane P. Wasko²  Fábio E. Severino²  Flávia T. Presti¹  Andréia B. Poletto¹  Cesar Martins¹ 

[1],Universidade Estadual Paulista Instituto de Biociências Departamento de GenéticaBotucatu SP ,Brazil

关键词: actin gene;    amino acid residues;    gene structure;    Nile tilapia;    nucleotide sequence;    Oreochromis niloticus;   

DOI  :  10.1590/S1415-47572007000600010

来源: SciELO

【 摘 要 】

An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.

【 授权许可】

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