Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa

Genetics and Molecular Biology

Alessandra A. De Souza¹  Marco A. Takita¹  Helvécio D. Coletta-filho¹  Maria Luisa P.n. Targon¹  Eduardo F. Carlos¹  Eliane C. Locali-fabris¹  Alexandre M. Amaral¹  Juliana Freitas-astúa¹  Ana Carla O. Silva-pinhati¹  Raquel L. Boscariol-camargo¹  Irving J. Berger¹  Carolina M. Rodrigues¹  Marcelo S. Reis¹  Marcos A. Machado¹ 

[1] ,Instituto Agronômico de Campinas Centro APTA Citros Sylvio Moreira Cordeirópolis SP ,Brazil

关键词: sweet orange;    EST;    CVC;    compatible interaction;   

DOI  :  10.1590/S1415-47572007000500023

来源: SciELO

【 摘 要 】

In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were associated with metabolism, protein modification, energy and transport facilitation. The majority of the up-regulated transcripts were associated with metabolism and defense response. Some transcripts associated with adaptation to stress conditions were up-regulated in plants with CVC and could explain why plants remain alive even under severe water and nutritional stress. Others of the up-regulated transcripts are related to defense response suggesting that sweet orange plants activate their defense machinery. The genes associated with stress response might be expressed as part of a secondary response related to physiological alterations caused by the infection.

【 授权许可】

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