Signaling pathways in a Citrus EST database

Genetics and Molecular Biology

Angela Mehta²  Marilia Santos Silva¹  Simone Guidetti-gonzalez¹  Helaine Carrer¹  Marco Aurélio Takita¹  Natália F. Martins² 

[1] ,Embrapa Recursos Genéticos e BiotecnologiaBrasília DF ,Brazil

关键词: Citrus;    cell signaling;    genomics;   

DOI  :  10.1590/S1415-47572007000500003

来源: SciELO

【 摘 要 】

Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

【 授权许可】

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