期刊论文详细信息
Genetics and Molecular Biology
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products
Marielle Cristina Schneider2  Bianca Pierina Carraro1  Doralice Maria Cella2  Rodrigo Rodrigues Matiello1  Roberto Ferreira Artoni1  Mara Cristina Almeida1 
[1] ,Universidade Estadual Paulista Instituto de Biociências Departamento de BiologiaRio Claro São Paulo ,Brazil
关键词: B chromosome;    constitutive heterochromatin;    karyotype;    meiosis;    Ag-NORs;   
DOI  :  10.1590/S1415-47572007000400023
来源: SciELO
PDF
【 摘 要 】

In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric region of all chromosomes, with the exception of the y p. Silver nitrate staining revealed one nucleolus organizer region (NOR) on the terminal region of the short arm of the second autosome pair. Silver staining of meiotic cells confirmed the NOR pattern detected in mitotic cells and revealed an argentophilous material on the Xy p. A cytogenetic comparison between the two populations of A. variegatus showed a statistically significant divergence (chi2 = 117.10; df = 1) in the number of aneuploid cells and a higher frequency of B chromosome in the population exposed to agrochemicals.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

【 预 览 】
附件列表
Files Size Format View
RO202005130147745ZK.pdf 162KB PDF download
  文献评价指标  
  下载次数:12次 浏览次数:15次