Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)

Genetics and Molecular Biology

Juliana Khouri²  Inês S. Resck¹  Marcio Poças-fonseca²  Thiago M.m. Sousa²  Luciana O. Pereira²  Adriano B.b. Oliveira²  Cesar K. Grisolia² 

[1] ,Universidade de Brasília Instituto de Ciências Biológicas Departamento de Genética e MorfologiaBrasília DF ,Brazil

关键词: Caryocar brasiliense;    anticlastogenicity;    antioxidant;    micronucleus;   

DOI  :  10.1590/S1415-47572007000300024

来源: SciELO

【 摘 要 】

The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had been treated with the highest dose of cyclophosphamide. When CHO-K1 cells were pretreated by adding 0.01, 0.05 or 0.1 mL of extract per mL of cell culture medium 24 or 48 h before bleomycin or cyclophosphamide there was a protective effect against chromosome breaks and a significant decrease in the mitotic index (a measure of cytotoxicity) of the CHO-K1 cells. The extract also had a protective effect against oxidative hydroxyl radical damage to 2-DR. This study suggests that C. brasiliense pulp aqueous extract has anticlastogenic potential, possibly due to its antioxidative properties.

【 授权许可】

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